Organ specificity and lactate-dehydrogenase activity. 1. The relative activities with pyruvate and 2-oxobutyrate of electrophoretically separated fractions.
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Organ Specificity and Lactate-dehydrogenase Activity. Differential Inhibition by Urea and Related Compounds.
1. The effect of urea on the lactate-dehydrogenase activities of human-heart and -liver tissue extracts and on crystalline ox-heart and rabbit-muscle enzyme have been determined. Similar studies on electrophoretically separated isoenzyme fractions have shown an inverse relationship between sensitivity to urea inhibition and electrophoretic mobility. 2. With pyruvate as substrate a sharp change ...
متن کاملRole of branched-chain 2-oxo acid dehydrogenase and pyruvate dehydrogenase in 2-oxobutyrate metabolism.
Purified branched-chain 2-oxo acid dehydrogenase (BCODH) and pyruvate dehydrogenase (PDH) had apparent Km values (microM) for 2-oxobutyrate of 26 and 114, with a relative Vmax. (% of Vmax. for 3-methyl-2-oxobutyrate and pyruvate) of 38 and 45% respectively. The phosphorylation state of both complexes in extracts of mitochondria from rat liver, kidney, heart and skeletal muscle was shown to infl...
متن کاملThe 2-oxo acid dehydrogenase complexes: recent advances.
The 2-oxo acid dehydrogenase complexes represent the classic examples of multienzyme complexes, a knowledge of whose structure and function has wide implications for our understanding of macromolecular assembly and organization and of protein structure and function. Each complex consists of multiple copies of three enzymes, termed El, E2 and E3. El is a 2-oxo acid dehydrogenase which has thiami...
متن کاملThe lactate dehydrogenases of hemopoietic cells.
By W. H. STARKWEATHER, H. H. SPENCER AND H. K. ScriocH M AMMALIAN LACTATE DEHYDROGENASE ( LDH ) can be separated into five active fractions.’4 The production of electrophoretic extremes, LDH-1 (H4 ) and LDH-5 ( M4 ), is controlled by the action of two genes.5’#{176}The intermediate fractions ( LDH-2, LDH-3, LDH-4 ) are considered hybrid forms of LDH-1 and LDH-5.5’” Assuming that the hybrids are...
متن کاملSome Properties of Human Spermatozoal Lactate Dehydrogenase
1. The presence of a characteristic lactate-dehydrogenase isoenzyme (LD-) in human, mouse and dog testis and in human spermatozoa has been confirmed by electrophoresis on cellulose acetate and on polyacrylamide gel. 2. The human spermatozoal isoenzyme exhibits a much higher affinity for 2-oxobutyrate than any ofthe five isoenzymes found in other tissues. Km values of 0-05mM for pyruvate and 0-1...
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عنوان ژورنال:
- The Biochemical journal
دوره 87 شماره
صفحات -
تاریخ انتشار 1963